Polymorphisms in genomic fingerprints generated by arbitrarily primed PCR(AP-PCR) can distinguish between slightly divergent strains of any organism. Sequence polymorphisms detected by genomic fingerprinting can be mapped genetically or used in phylogenetic and population studies, but too many factors affect the AP-PCR result. Sometimes, it is difficult to obtain a perfect genomic fingerprints are difficult. So an optimization project with minimum times of test is needed. The optimization of the amplified conditions included concentration of Mg²⁺，concentration of DNA, concentration of primers were reported. The results show that the optimum amplified condition of AP-PCR are 2 mmol/L MgCl₂, 50 ng of DNA and 0.3 μmol/L of primers. Under these conditions the AP-PCR productions are richness and the most clear.