对检测人结肠癌组织端粒酶活性的端粒酶重复序列扩增法(TRAP)进行了研究.当Mg2+浓度为1.8 mmol/L、退火温度为55℃时,能得到可靠的检测结果.影响检测结果可靠性的另两个因素是抽提物的反应用量和阳性片段的污染.
A TRAP assay on human colonic tumor telomerase activity is reported. The reliable result is obtained when 1.8 mmol/L Mg2+ and 55℃ annealing temperature are adopted. The other two factors affecting reliability are quantity of the extract and contamination of the positive DNA fragments in the reaction.
顾少华,江广平,曹根涛,盛继群,刘建平,戴建凉,林卿,林渊,李实忠,刘为平,谢毅.检测人结肠癌组织端粒酶活性的TRAP方法研究[J].生物化学与生物物理进展,1999,26(1):72-75 GU Shao-Hua, JIANG Guang-Ping, CAO Gen-Tao, SHENG Ji-Qun, LIU Jian-Ping, DAI Jian-Liang, LIN Qing, LIN Yuan, LI Shi-Zhong, LIU Wei-Ping, XIE Yi. A TRAP Assay on Human Colonic Tumor Telomerase Activity[J]. Progress in Biochemistry and Biophysics,1999,26(1):72-75
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