The effects of gel or electrode buffer strips using agarose with different electroendosmosis of 0, 0.03, 0.08, 0.20 mr on electrophoresis were studied. It was shown that only agarose without electroendosmosis can be used for isoelectric focusing. The electrod buffer strips with different electroendosmosis did not affect SDS PAGE significantly but they affect Native PAGE in certain extent. The higher the electroendosmosis was, the more difficultly it ran.